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Citrus tristeza virus (CTV), a member of genus Closterovirus, is an important pathogen which has destroyed more than one million citrus trees in India till date. Field surveys were carried out in major citrus growing regions of Vidarbha region (Nagpur, Amravati, Akola and Wardha). Under field conditions, the CTV infected trees of citrus exhibited stunted growth, vein clearing, vein flecking, chlorosis with decline and reduced leaf size. The CTV incidence of 13.5 % in sweet orange followed by 10.0 % in mandarin and 6.0 % in acid lime was reported during survey. Among three cultivars surveyed sweet orange found higher susceptible to CTV. In the present investigation biological indexing was done by graft inoculating acid lime as a indicator host with tissues from suspected citrus trees. Highly predictable symptoms were developed on indicator host acid lime viz., seedling yellow after 8 week inoculation.
Key words: CTV, Survey, Biological indexing.