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This study was conducted at Haji Roshan Suleman Hospital and College Tandoadam (January, 2015 to March, 2016). Blood donors at the Hospital were used as subjects to study the frequency of Anti-HCV, HBsAg, Syphilis and HIV. A population of 2200 blood donors were evaluated for Anti-HCV, HBsAg, Syphilis and HIV. Patients were subjected to Immuno-chromatography (ICT method) for the qualitative detection of antigens and antibodies respectively. Enzyme Linked Immuno Assay (ELISA) was used to screen for antigens and antibodies of HBV and HCV, respectively. Collection of the blood samples was conducted under the supervision of a Consultant Pathologist by Technicians of the Hospital Laboratory. Blood grouping via both forward and reverse methods was conducted. 263 patients out of a total population of 2200 suffered through Transfusion-transmitted Infection (11.95 %). The prevalence for Anti-HCV, HBsAg, Syphilis and HIV antibodies was 149 (6.77 %), 40 (1.81 %), 74 (3.36 %) and 3 (0.13 %) respectively. Blood group O+ve is more prevalent (39.54 %). This sky high prevalence of HCV infection in blood donors espouses the implementation of stern screening policies for donors and public awareness campaigns about preventive measures to bring down the escalating frequency of this infection as well as other transfusion transmissible infections.

Key words: Transfusion Transmitted Infections, HCV, HBV, Syphilis, HIV.