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An efficient and cost effective micro propagation protocol using MS medium was developed for
Catharanthus roseus and Bacopa monnieri. Shootlets were regenerated from nodal explants of stem
through auxiliary shoot proliferation.
In Catharanthus roseus, the induction of multiple shoots from nodal segments were premier in MS
medium supplemented with 0.5 mg/l BAP ± 1mg/l NAA. and maximum rooting was recorded on MS
medium with 5.0 mg/l IBA.
In Bacopa monnieri, the induction of multiple shoots were highest in MS medium supplemented with 0.5
mg/l BAP + 2.0 mg/l Kn and 0.5 mg/l Kn+1.0 mg/l BAP. and highest rooting was recorded on MS
medium supplemented with 2.0 mg/l IBA. Leaf petiole explants were used for the purpose of callus
induction. Best growth was observed in MS medium supplemented with 0.25 mg/l 2, 4-D+ 0.5 mg/l Kn
and 0.25 mg/l 2,4-D+ 0.1mg/l BAP.
Key words: Catharanthus roseus, Micropropagation, Medicinal plant, Callus induction.