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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2018 , Volume 6, Issue 2
Page No. : 806-809
Article doi: : http://dx.doi.org/10.18782/2320-7051.5984
Effect of pH on Callus Induction and Shoot Regeneration from Cotyledon and Leaf and Hypocotyl Explants of Tomato
Rizwan Rashid1*, Bhat J. A2., Mohd Iqbal Bhat3 and Bilal Ahmad Bhat4
1KVK - Zanskar Sher-e-Kashmir University of Agriculture Science and Technology (K) –Srinagar, India
2Department of Agriculture Kashmir
2KVK - Budgam Sher-e-Kashmir University of Agriculture Science and Technology (K) –Srinagar, India
3KVK - Bandipora Sher-e-Kashmir University of Agriculture Science and Technology (K) –Srinagar, India
*Corresponding Author E-mail: rizwan60@gmail.com
Received: 10.11.2017 | Revised: 26.12.2017 | Accepted: 2.01.2018
ABSTRACT
To standardize medium pH for in vitro callus induction and shoot regeneration from cotyledon and leaf explants of genotype Punjab Upma and IPA-3 of tomato, the explants were cultured on the MS medium which was maintained at wide range of pH (4.5-7.0). Callus induction and shoot regeneration were significantly affected by medium pH. The callus induction and shoot regeneration were affected with lower pH (4.5-5.0) and higher (6.0-7.0) medium pH significantly. Among two explants used cotyledons shows the maximum callus induction as well as shoot regeneration. The best callus induction and shoot regeneration occurred in the medium pH 5.5-6.0.
Key words: Callus induction, Shoot regeneration explants.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.5984
Cite this article:
Rashid, R., Bhat, J.A., Bhat, M.I. and Bhat, B.A., Effect of pH on Callus Induction and Shoot Regeneration from Cotyledon and Leaf and Hypocotyl Explants of Tomato., Int. J. Pure App. Biosci. 6(2): 806-809 (2018). doi: http://dx.doi.org/10.18782/2320-7051.5984
