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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2025 , Volume 13, Issue 3
Page No. : 10-17
Article doi: : http://dx.doi.org/10.18782/2582-2845.9157

<p>Studies on the Association of PKLR Gene Polymorphism with Milk Production Traits in Cattle</p>

Chavhan Snehal¹, P V Yeotikar^2*, P B Ghorphade³, M J Sanap⁴ , L A Pangaonkar⁵ , S. Sajid Ali⁶  and M P Sakhare⁷ 

Maharashtra Animal and Fishery Sciences University, Nagpur, (Maharashtra)

 ABSTRACT

The present study aimed to investigate the association of PKLR (Pyruvate Kinase expressed in Liver and Red blood cells) gene polymorphism with milk production traits in thirty lactating Red Kandhari Cattle from the Marathwada region. The present investigation included PKLR gene polymorphism and its association with milk production traits, viz, Milk yield (Kg/day) and milk constituents, viz, Milk Fat per cent, SNF per cent, Protein per cent, Lactose per cent, Water per cent, and Salt per cent in Red Kandhari cattle. The isolated DNA of interest (region 3: g.15350898T>C for exon 10, which was CTTCGAG) from blood samples of Red Kandhari cattle was amplified (149 bp, 148 bp, and 71 bp PKLR gene) by PCR  and the PCR products were analyzed by SSCP for PKLR gene polymorphism. The data from SSCP analysis were statistically analyzed using the HW-equilibrium method to detect gene and genotype frequencies. The milk yield and milk constituent data were compared with the reviewed literature. The results of the investigation of PKLR gene polymorphism in Red Kandhari cattle showed that all three primers showed the same pattern of these single-stranded DNA bands having the same migration patterns, confirming the monomorphism in all the samples of experimental animals. The known mutant alleles at PKLR gene were not observed in the present study. In Red Kandhari cows, the gene and genotypic frequencies of the PKLR gene were analyzed. The frequency of the C allele was observed to be zero, while the frequency of the T allele was 1. Consequently, the genotypic frequencies were as follows: 0.0 for CC, 0.0 for CT, and 1.0 for TT. The mean ± SEM values of milk yield (kg/day) of Red Kandhari cattle were observed to be 2.62±0.03. It is concluded that, In the present study, no polymorphism or association was observed with milk production traits in Red Kandhari cattle.

Keywords: SSCP, Milk production traits, Red Kandhari cows, PKLR gene.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2582-2845.9157

Cite this article:,

Cite this article: Snehal, C., Yeotikar, P. V., Ghorphade, P. B., Sanap, M. J., Pangaonkar, L. A., Sajid Ali, S., & Sakhare, M. P. (2025). Studies on the Association of PKLR Gene Polymorphism with Milk Production Traits in Cattle, Ind. J. Pure App. Biosci. 13(3), 10-17. doi: http://dx.doi.org/10.18782/2582-2845.9157