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Aloe barbadensis Mill. was used as an alternative medium for growing A. xylinum culture to produce
Microbial Cellulose (MC). A one year and a six months old A. barbadensis Mill. leafs were mixed to
produce A. barbadensis Mill.-medium (ABM-medium). The mixture ratio of the one year and six months
old Aloe barbadensis Mill. are 100%:0%; 75%:25%; 50%:50%; 25%:75%; 0%:100% respectively.
Distilled water was used as control. These media were then fermented for seven days in 28 0C. The dry
and wet weight data of the produced MC were collected and analyzed using one way-ANOVA. The result
showed that ABM-media produced more MC than the control (P ≤ 0,05) and the optimum ratio of the
ABM-medium is 0% old:100% young based on the wet and dry weight. This result was then used in the
second experiment. Initial pH of 3, 5, 7 and 9 were combined with glucose concentration of 0% (control);
2,5%; 5%; 7,5%; 10%; 12,5% and 15% using factorial ANOVA design to get the optimum combination of
both in the ABM-medium. After seven days of fermentation in 28 0C
the result showed that the optimum combination of all is pH 3 with 15% glucose (P ≤ 0.05) with the
highest wet and dry weight of MC.
Keywords: Acetobacter xylinum, Microbial cellulose, Aloe barbadensis Mill., ratio, medium.