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Research on Xylanase enzyme has markedly increased due to its potential applications in several
industries include pulping and bleaching processes, where it is using cellulose free preparations, textile
processes, the enzymatic saccharification of lignocellulosic materials and waste treatment. The impact of
ultra violet radiations on xylinase producing ability of Bacillus subtilis was studied. Maximum enzymatic
activity (crude) upto 17 folds were obtained from mutant strain exposed to 60 minutes ultra violet
irradiation in comparison to parent strain. Purified enzyme showed molecular weight of 38 KDa and 44
KDa by SDS-PAGE in both parent and mutant strains. The SDS-PAGE of mutant strain also showed the
presence of other bands of higher molecular weight indicating the presence of additional proteins
resulting due to UV exposure, leading to the higher production of xylinase. Result of the Agarose gel
electrophoresis of genomic DNA of parent and mutant strain showed that changes in the gene expression
due to impact of UV does not change the genome of mutant strain of Bacillus subtilis.
Keywords: Bacillus subtilis, xylinase, enzymatic activity, SDS-PAGE.