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Pesticide degradation is the process by which pesticide is transformed into a benign substance that
is environmentally compatible with the site to which it was applied. In the present work, twelve
pesticide resistant bacterial isolates capable of tolerating four pesticides such as chlorpyrifos,
malathion, chlordane and chlorothalonil were obtained by spot assay enrichment technique from
field soil. Among them Klebsiellasps (K1, K2 and K4), Pseudomonas sps(P3) and Bacillus sps(B4)
showed more resistance and were used for further studies. We found that among those organisms,
the three Klebsiellasps were the most effective pesticide tolerating organisms and also found that the
pesticide tolerating property was plasmid origin.
Keywords – Pesticides, spot assay, Klebsiellasps, plasmid.