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Proteolytic bacteria are most important for industries such as food and fermentation thus isolating
and manipulating pure cultures from various source has important for various biotechnology
industries Different varieties of bacterial species (SB1,SB2,SB3,SB4)are isolated from “Dairy
sludge” and all the isolates will be screened for their proteolytic activity using skimmed milk agar
plates, in which SB3 are found to be protease producing lactobacilli .Production of alkaline
protease is carried out in modified production medium , centrifuged and supernatant was taken as
crude enzyme. Industrial applications as stain buster ,as it used for the removal of blood stain
which shows the maximum removal of stains by its proteolytic activity.
Keywords: Dairy sludge, skim milk agar, alkaline protease, stain buster.