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Hemidesmus indicus is an important medicinal plant which is widely used in traditional and modern
medicinal systems. In order to identify the genetic similarity and diversity among the collected
accessions of H. indicus, Randomly Amplified Polymorphic DNA(RAPD) analysis was carried out. A
total of 12 accessions growing in the Botanical garden of Department of Botany, Osmania
University have been taken up for the study and the RAPD markers have been identified for
them.The dendrogram was generated by UPGMA whichshowsa wide range of variability among the
accessions.The maximum genetic similarity among the collected species was 64% which was
calculated by Jaccard’s coefficient. These results could be very useful to study and manage the
genetic resources of this important medicinal plant.
Keywords: Hemidesmusindicus, accessions, RAPD, dendrogram, genetic similarity.