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Phytochemical screening was carried out on the aerial parts of Eleusine indica with the aim of
establishing the ethnomedicinal claims on the vegetative part. The results revealed the presence of
alkaloids, flavonoids, cardiac glycosides, tannins and acidic compounds. The chloroform and
methanol extracts had antibacterial and anti fungal activities against the test organisms – S.aureus,
E. coli, Enterobacter aerogens, P. Vulgaris, Streptococcus specie, Bacillus specie, Pseudomonas
aerogenes, Klebsiella aerogenes, Aspergillus flavus, Aspergillus niger and Candida albicans. The
minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the two
extracts confirmed that they were active at very low concentrations. Pure compounds from the
extract gave yellow crystalline solids which were characterized using spectroscopic instruments
such as GC-MS, UV, FTIR, H1- NMR and C13 – NMR. Hexadecanoic acid, [[[(2-
aminoethoxy)hydroxyphosphinyloxy]methyl]-,2 –ethanediylester was the pure chloroform extract
while Hexadecanoic acid (C16H32O2)is the pure methanol extract.
Keywords: Phytochemical/Antimicrobial analysis, Chloroform/Methanol extracts, Structural elucidation.