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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2015 , Volume 3, Issue 2
Page No. : 123-127
Article doi: : http://dx.doi.org/10.18782
Plantlet regeneration from Callus culture of various explants of Winged bean Psophocarpus tetragonolobus variety NS 122
D.S.R. Naik1*, Bylla Prasad2 ,Gandhi Nemali1 and A. Seetaram.Naik1
1Department of Botany, Kakatiya University, Warangal, Telangana State, India-506001.
2Department of Biotechnology, Kakatiya University, Warangal, Telangana State, India-506001
*Corresponding Author E-mail: ravinaik0805@gmail.com
ABSTRACT
Protein content in Psophocarpus tetragonolobus is comparable to soya bean, higher that of other
pulses, it is high yielding legume crop in tropical country like India. An in vitro plantlet regeneration
protocol was developed from callus cultures of leaf, petiole and internode in P. tetragonolobus with
combination of 2,4-D & Thidiazuron. Leaf callus show maximum percentage of plantlet
regeneration in combination with 2,4-D + TDZ (1.5+0.5) petiole callus show moderate percentage
of plantlet regeneration in combination with 2, 4-D+ TDZ, (1.5+0.75), internode callus show
minimum percentage plantlet regeneration in combination with 2, 4-D+TDZ, (1.5+1.2). Leaf explant
show maximum plantlet regenerated percentage (70-80) in combination with 2,4,D & Thidiazuron
when compared to other explants such as petiole and internode.
Keywords: Callus, Plantlet regeneration, Psophocarpus tetragonolobus, explants
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 3 (2): 123-127 (2015)
