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Somatic embryos Development from Leaf and Internode explants of Lathyrus sativus L. Lathyrus
sativus L. commonly known as grass pea. Here in the present study we developed an efficient
micropropagation protocol through somatic embryogenesis from leaf and intermodal explants of
Lathyrus sativus. Murashigae-Skoog medium supplemented with 2,4-D (1.0mg/l) and
(BAP)benzylaminopurine (5 mg/l) resulted in the production of green embryogenenic callus with
clearly differentiated somatic embryos with frequency of plant conversion rate from leaf and
internode explants was50±0.8 and 53±0.5 respectively. The regenerated shoot were rooted on root
induction medium, transplanted to pots and later transferred to field. The survival percentage of the
transplanted plants was 33±0.1for Internode and 24±0.6 for leaf explants. Therefore, this protocol
could be useful for micropropagation of Lathyrus sativus.
Keywords: Grass pea, Somatic Embryogenesis, Leaf and Internode explants, Micropropagation, MS Medium.