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Syngonium podophyllum‘Mini Pixie’ is an ornamental dwarf plant having green leaves with creamwhite
centers. Shoot tips of such plants were cultured on Murashige and Skoog’s medium
supplemented with individual and combined plant growth regulators such as Zeatin and Indole-3-
acetic acid at 4.56 to 11.41μM concentrations. Clumps of PLBs were induced from the explants, on
media containing combined growth regulators after twelve weeks of growth period. Subculture of
PLBs on same medium or incorporating 1-Naphthalene acetic acid as an auxin, showed
differentiation of stunted shoots both in dark and light conditions. Healthy plantlets were developed,
when clumps of shoots were transferred to basal media without any plant growth regulators. Shoot
multiplication from in vitro grown shoot tips was also observed on media supplemented with 1 mg/l
of each 6-Benzylaminopurine and 1-Naphthalene acetic acid. The in vitro regenerated plants were
harvested and maintained in closed glass containers with sand or stones and 20ml drinking water
for making indoor decorative gift articles based on ornamental plants. This protocol of PLB
induction, their germination, rapid shoot multiplication and plant regeneration can be applicable to
the production of artificial seeds, micropropagation, transformation studies and also making
innovative gifts.
Keywords: Protocorm like bodies, Micropropagation, Regeneration, Syngonium ‘mini pixie’, Gift articles