-
No. 772, Basant Vihar, Kota
Rajasthan-324009 India
-
Call Us On
+91 9784677044
-
Mail Us @
editor@ijpab.com
Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2015 , Volume 3, Issue 2
Page No. : 534-541
Article doi: : http://dx.doi.org/10.18782
Production and biochemical characterization of alkaline, thermostable xylanase secreted by recombinant Escherichia coli
Girish K. Goswami1* and Seema Rawat2
1Professor, Department of Biotechnology, C U Shah Institute of Life Science, C U Shah University, Wadhwancity, Surendranagar, Gujarat, India
2Associate Professor, Department of Physiology, C U Shah Medical College, Surendranagar, Gujarat, India
*Corresponding Author E-mail: girishkgoswami@gmail.com
ABSTRACT
Xylanase gene was expressed in Escherichia coli. The enzyme production was induced in E. coli and
liquid broth was used for enzyme estimation and characterization. Total three positive clones were
used in the study naming sample 1, 2 and 3. Sample no 2 was found the best in expression of
xylanase. To compare all the various parameters of the recombinant enzyme all 3 sample were
studied simultaneously. The enzyme showed maximum activity at alkaline pH 7.5 and high
temperature 60 °C respectively. The enzyme showed good thermal stability with half life of 3 hrs at
60 °C. Molecular weight of recombinant xylanase (determined by SDS-PAGE) was found 22 kDa.
Km and Vmax of the recombinant xylanase was found to be 8.17 mg/ml and 3.7 IU/ml, respectively.
Keywords: Xylanase, E. coli, Birchwood, endo-1,4-β-xylanases
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 3 (2): 534-541 (2015)
