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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2015 , Volume 3, Issue 3
Page No. : 10-16
Article doi: : http://dx.doi.org/10.18782
In vitro Micropropagation of a Valuable Medicinal Plant, Piper crocatum
Zuraida A.R.1*, Erny Sabrina M.N.2, Mohd Shukri M.A.2,Razali M.2, Norma H.3 and Wan Zaliha W.S.4, and Ayu Nazreena O.1
1Biotechnology Research Centre, MARDI HQ, Persiaran MARDI-UPM, 43400 Serdang Selangor, Malaysia
2Strategic Resources Research Centre MARDI HQ, Persiaran MARDI-UPM
3Horticulture Central, MARDI HQ, Persiaran MARDI-UPM, Serdang Selangor, Malaysia
4School of Food Science and Technology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia 43400 Serdang Selangor, Malaysia
*Corresponding Author E-mail: azuraida@mardi.gov.my
ABSTRACT
A micropropagation protocol in valuable medicinal plant, Piper crocatum was developed which may
assist in promote the industry herb in Malaysia. In the present research effect of plant growth
regulators (PGRs) on shoot initiation and propagation of Piper crocatum was investigated. Shoot
was initiated from internodes and apex shoots on MS medium supplemented plant growth regulator.
Three type of anti-browning, proline, PVP and charcoal were tested to reduce browning problem.
Internodes showed better response for shoot induction than apex shoots. MS media containing 5
mg/L BAP, 0.5 mg/L 2, 4-D supplemented with charcoal was the most effective in shoot initiation,
proliferation and showed less in browning. The developed complete plantlets with root were
successfully acclimatized and established in glass house and the final survival rate was 70%.
Key words: Piper crocatum, Micropropagation, Medicinal Plant, plant growth regulator.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 3 (3): 10-16 (2015)
