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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2015 , Volume 3, Issue 3
Page No. : 105-112
Article doi: : http://dx.doi.org/10.18782
Cloning of new xylanase gene from Bacillus brevis in to Escherichia coli
Girish Kumar Goswami*
C U Shah Institute of Life Science, C U Shah University, Wadhwancity, Surendranagar, Gujarat, India
*Corresponding Author E-mail: girishkgoswami@gmail.com
ABSTRACT
A gene for xylanase was isolated from Bacillus brevis and cloned in Escherichia coli BL21 host for
heterologous gene expression. The xylanase gene was amplified by specifically designed primers in
pUC vector while the PCR product was cloned in pET29a vector. Sequencing of the gene (GenBank
accession number: bankit1383328 HQ179986) showed that it belongs to family 11 xylanases. The
temperature and pH optima of B. brevis xylanase were found to be 55°C and 7.0, respectively. This
xylanase gene was successfully expressed in BL21 with 30 IU/ml activity (~ 2 times higher than
native host).
Keywords: β-1, 4 Endo-xylanase, Bacillus brevis, Cloning, Vector
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 3 (3): 105-112 (2015)
