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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2015 , Volume 3, Issue 3
Page No. : 124-128
Article doi: : http://dx.doi.org/10.18782
Phylogenetic analysis of the Fusion (F) and Haemagglutinin (H) genes of Canine Distemper Virus from field isolates in Tamil Nadu
Aarthi Komandur Seshadri1*, Sherrill Wesley Josewin2 and Nita Elizabeth Jojo2
1Dept. of Animal Biotechnology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
2Dept. of Biotechnology, St. Joseph’s college of engineering, Chennai, Tamil Nadu, India
*Corresponding Author E-mail: ksaarthi@outlook.com
ABSTRACT
Canine Distemper Virus (CDV) is a highly transmissible viral pathogen that causes the lethal canine
distemper disease which has high mortality rates. In the present study, 15 clinical specimens, 8
ocular swab specimens, 6 blood specimens and 1 nasal swab specimen were collected from
suspected dogs. The Fusion gene and Haemagglutinin gene regions of CDV were amplified using
reverse transcriptase-PCR. Subsequently partial gene sequencing was performed to compare the
genetic variation among the field isolate and vaccine strain. The obtained sequences were also
compared with the reference CDV sequences available in the Gen Bank to establish the phylogenetic
lineage. The results obtained suggested that CDV is prone to evolution and that a different CDV
lineage may be present in Tamil Nadu. Variations in the amino acid site in the F and H gene regions
indicated the need for the development of new candidate vaccines in Tamil Nadu.
Key words: Canine distemper virus, Onderstepoort strain, Fusion gene, Haemagglutinin gene, Phylogenetic analysis
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 3 (3): 124-128 (2015)
