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This study focused on to evaluate effects of oral as well as intraperitoneal administration of various
doses of refinery sludge on the bone histology in a guinea pig model. Adult guinea pigs were
randomly selected to which refinery sludge was administrated in a dose range of 1.5-6.0mg/kg body
wt. for a period of 3 weeks. The bone of interest from this animal model were fixed and decalcified.
After being dehydrated with ascending grades of ethanol and xylene,the specimens were embedded
in paraffin. These specimens were cut into serial sections 5 μm thick each in the frontal plane
passing through the midline between the medial and lateral menisci. These sections were subjected
to haematoxylin and,safranin-O-fast green, Masson’s trichrome and toluidine blue staining.
Histopathological analyses showed that refinery sludge insulted guinea pigs showed decrease in
bone cell population density and cartilage degeneration and necrosis. Dose dependent and time
dependant refinery sludge insulted might have developed lesion in the bone of guinea pigs,could well
be inferred from this study,however demands further evaluation.
Key words: sludge,guinea pig,histopathological,bone,cartilage