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Introduction: Cardiovascular disease (CVD) is an important cause of mortality and morbidity in
India. Analysis of Coronary heart disease by cross sectional and epidemiological studies performed
over past 50 years reveals that (CVD) is increasing both in urban and rural areas. There is growing
body of evidence for role of free radicals in mediating myocardial injury during myocardial
ischemia and in particular during the phase of myocardial reoxygenation. Aim of the study: To
study the protective effect of antioxidant Glutathione on hematological parameters in isoproterenol
induced myocardial infarction in wistar male rats. Materials and methods: wistar male rats were
randomly divided into four groups namely Control (G1), Glutathione (G2), Isoproterenol (G3) and
Isoproterenol+Glutathione(G4). Glutathione treated group-received Glutathione (200mg/kg body
wt) orally for 30 days. Myocardial infarction was induced in rats by isoproterenol administration
(100mg/kg) subcutaneously (sc) at an interval of 24 hrs on 31st and 32nd day. Hematological changes
were assessed from 24 hrs after the last dose of isoproterenol. Results: There was decrease in RBC
count, WBC count, Platelet count, Hb%, Lymphocyte count, Eosinophil count and increase in
Neutrophil count in G4 rats as compared to G3 rats Glutathione pre treatment preserved the
changes to near normal. Conclusion: Glutathione may provide cardio protection under conditions
of I-R, by virtue of its antioxidant properties, and may prevent oxidant-mediated damage of the
cardiomyocyte membrane and subsequent intracellular Ca2+ overload.
Key words: Glutathione, Hematological Parameters, Isoproterenol