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Preliminary phytochemical and antibacterial screening was conducted using methanol ethanol and
chloroform extracts of Eclipta prostrata L. and Sphaeranthus indicus L.Preliminary phytochemical
screening was done on Methanol extract, Ethanol extract and chloroform extract in which the
methanolic extract revealed maximum number of phyto-constituents. This study revealed the
presence of Alkaloids, flavonoids, Tannins, Terpenoids, Steroids and Glycosides.In antibacterial
screening both the plants exhibited significant activity against Shigella boydii, Escherichia coli,
Klebsiella pnuemoniae, Pseudomonos Sps, Salmonella paratyphi A.The secondary metabolites are
responsible for the medicinal activity of studied plants. This study concluded that E.prostrata L. and
Sphaeranthus indicus L. have sufficient antibacterial activity due to the presence of secondary
metabolites.
Key words: Phyto-constituents, antibacterial activity, Secondary metabolites, Asteraceae