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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2015 , Volume 3, Issue 4
Page No. : 1-9
Article doi: : http://dx.doi.org/10.18782
Larvicidal activity of Piper tuberculatum extracts on the tobacco budworm, Heliothis virescens Fabr. (Lepidoptera:Noctuidae) under laboratory conditions
Lisset J. Monsalve-Asencio1, Nathali L. Mendoza-Frías1, Consuelo Rojas-Idrogo1, Massuo J. Kato2, Jorge Saavedra-Díaz3 and Guillermo E. Delgado-Paredes1*
1Facultad de Ciencias Biológicas, Universidad Nacional Pedro Ruiz Gallo, Ciudad Universitaria, Juan XXIII No 391, Lambayeque, Perú
2Instituto de Química, Universidade de São Paulo, CP 26077, 05599-970, São Paulo-SP, Brazil
3Facultad de Agronomía, Universidad Nacional Pedro Ruiz Gallo, Ciudad Universitaria, Juan XXIII No 391, Lambayeque, Perú
*Corresponding Author E-mail: guidelg2001@yahoo.es
ABSTRACT
Plant extracts and isolated metabolites have long been a subject of research due to the increased
concern for adverse effects of conventional insecticides on human health and environment. The
larvicidal activity of the neotropical “matico” Piper tuberculatum on the tobacco budworm,
Heliothis virescens was evaluated by contact bioassays. The secondary compounds were extracted
from mature spikes with fruits and seeds of wild plants and in vitro 12-months-old plants of P.
tuberculatum.CH2Cl2:MeOH (2:1) extracts from mature spikes caused 76.6 and 83.3% mortality
when doses of 0.012 mg/μL were applied to H. virescens in 76 and 120 h of exposure, respectively,
with LD50 0.007 mg/μL and LD90 0.033 mg/μL, in 76 h of exposure. The CH2Cl2:MeOH (2:1)
extracts from in vitro plants caused 56.6% mortality when doses of 0.012 mg/μL were also applied
in 76 h of exposure with LD500.011 mg/μL and LD90 0.046 mg/μL. The potential value of extracts
derived from P. tuberculatum as efficient insecticides against H. virescens is discussed.
Keywords: CH2Cl2:MeOH extract, in vitro propagation, insecticide, larval susceptibility, lethal concentration.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 3 (4): 1-9 (2015)
