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Pathogenic bacterial resistance against antibiotic was one of major problem in medical concern.
Staphylococcus aureus or Methicillin Resistant Staphylococcus aureus (MRSA) is one of those
pathogenic bacteria. This research purposes to discover the new antibacterial substance using strain
indentified as Nocardia sp. which belongs to Actinomycetes. Bacterial isolate was collected from
Udayana University Microbiology Laboratory, Indonesia. Antagonistic evaluation using pour plate
method in Nutrient Agar (NA) medium shows Nocardia sp. 1 strain has the highest result of
inhibition zone test (22.7 mm long) against MRSA compared to 6 other strain. Nocardia sp. 1 strain
filtrate tested on MRSA has a MIC level of 2%. Later identification of chemicals substance contained
in Nocardia sp. 1 strain filtrate detects substances such as 3,5 -Dichloro- 2 pyridone, Benzeneacetic
acid, Hepatdecane, Phenol. 2.4-bis(1.1-dimethylethyl), Dodecanoic Acid Methyl Ester,
Hexadecanoic Acid Methyl Ester, 1-(+)-Ascorbic Acid 2.6 Dihexadecanoate.
Key words: Antibacterial, Actinomycetes, Methicillin Resistant Staphylococcus aureus, MIC.