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A roving survey of different redgramfields for begomovirus incidence was conducted in the Kolar
district of Karnataka during kharif 2014-15. Results indicated that disease incidence was ranged
from 1-5 percent.The infected plants initially showed, small yellow speck on the newly developed
leaves later, these specks coalesced and formed larger yellow patches. The infected plants
showedstunted growth and symptoms and there was no production of any fruits. PCR product of
520bp of coat protein region was amplified using deng primers with infected samples of redgram.
The nucleotide sequences of viral CP shared maximum nucleotide identity (98%) with previously
identified horse gram yellow mosaic virus (HYMV) strains of southern India followed by 87%
identity with Mung bean yellow mosaic virus (MYMV). Phylogenetic analysis of begomoviruse’s CP
nucleotide sequences grouped into HYMV infecting legumes and MYMV infecting legumes which are
previously described as members of begomoviuses from different geographical locations. The virus
infecting redgram in Karnataka formed a separate cluster with begomoviruses (HYMV and MYMV)
infecting legumes indicated that, virus associated with redgram yellow mosaic disease is a closest
relative of HYMV and MYMV.
Keywords: Redgram, Begomovirus, Disease incidence, Sequencing, Phylogeny