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In the present study, the potential of Bacillus licheniformis MTCC 429 to decolourize reactive blue
dye used in textile industry was evaluated. The influence of different incubation time, pH,
temperature, inoculum concentration and carbon sources was studied to find the optimum conditions
required for maximum decolourization of reactive blue dye using B. licheniformis MTCC 429.
Incubation time of 24 hrs, with pH 7, temperature 35°C and 5% inoculum were observed to be the
optimum decolourizing conditions. Glucose showed the maximum dye decolourization when
compared to other carbon sources tested. The fruit wastes such as sapota and apple peel waste
extracts were tested as substrate for the growth and dye decolourization ability of the bacteria. The
sapota peel waste extracts served as better substrate which exhibited 91% decolourization ability
when compared to apple waste. The results of the present study evidenced the potential of B.
licheniformis MTCC 429 to be used in the biological treatment of textile effluents containing
recalcitrant textile dyes.
Key words: Bacillus licheniformis MTCC 429, reactive blue, decolourization, fruit waste