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Microbial fuel cell (MFC) has become one of the sustainable energy technologies for waste water
treatment purposes in the recent years. It combines waste water treatment and electricity generation
together to achieve energy balance. Four types of locally isolated bacteria (Escherichia coli,
Pseudomonas aeruginosa, Bacillus sp, and Klibsella pneumonia) were tested for their ability to
produce electricity. Two kinds of municipal wastewater were used; from aerobic and anaerobic
treatment stages, while providing the system with nutrient glucose continuously. Results showed that
the highestvalueof the voltage generated when using the bacteria Bacillus sp. It gave 0.6 Volt and
lasted for two daysat room temperature. The smallest value was recorded when aerobic treatment,
and it was 0.3 Volt and lasted for three days at room temperature and pH of 8.6.
Key words: Bacillusspp.; Microbial Fuel Cell and Wastewater