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In this study, Cholesterol degrading Lactobacillus was isolated from butter milk sample. The growth
performance of Lactobacillus on MRS agar was identified by the colonies were formed due to the
utilization of Lactose and by various biochemical tests. Lactobacilli are resistant to various
antibiotics and bile salt. Growth performance of selected probiotic in various pH (such as 4, 7 & 9),
Temperature (such as 40C, 370C, 500C), and various concentration of Bile salt (such as 0.1g, 0.2g,
0.3g,0.4g and 0.5g ) was detected. In vitro study of cholesterol degradation by probiotic was carried
out and it was based on the growth performance and percentage of cholesterol removed from liquid
cholesterol. The result of in vitro study reveals Lactobacillus showed the better degradation (21.5%)
of cholesterol degraded in liquid cholesterol (at 600 μg/ml).
Key words: Cholesterol, Lactobacillus, Probiotics, Bile salt and MRS medium.