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Nanobiotechnology gives emphasis for the synthesis of nanoparticles using living organisms such as
microorganisms, plant extracts or plant biomass in an eco-friendly way. Among the various agents used
for nanoparticle synthesis, plants have found important application. The biomolecules found in plants
induce the reduction of Ag+ ions from silver nitrate to silver nanoparticles (AgNPs). The aqueous leaves
extract of Terminalia chebula was used as reducing and stabilizing agent for the synthesis of silver
nanoparticle. Synthesized nanoparticle is confirmed by the change of color from transparent yellow to
dark brown indicates the formation of silver nanoparticles. UV-Vis absorption spectroscopy was used to
monitor the quantitative formation of silver nanoparticles. The UV visible spectrum of colloidal solutions
of SNPs was 199.00nm. The antimicrobial assays were done on human pathogens like Escherichia coli,
Staphylococcus aureus, Salmonella typhi and Klebsiella pneumonia. The zone of inhibition of synthesized
silver nanoparticles were found to be 18 mm for Salmonella typhi, 16 mm for S. aureus, 15 mm for E. coli
and 20 mm for K. pneumoniae. The plant based route could be considered to be an environmental
friendly, safe and economic biological method for the silver nanoparticles production.
Keywords: Silver nanoparticles, Medicinal plants, Green synthesis, Ecofriendly.