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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2016 , Volume 4, Issue 1
Page No. : 109-114
Article doi: : http://dx.doi.org/10.18782/2320-7051.2185

Enhanced Production of Cellulolytic Enzymes from Immobilized Cells of Aspergillus niger

M. Siddiqui^1*, N. Siddiqui² and R. Varma¹

¹Department of Botany, SNGGPG College, Shivaji Nagar, Bhopal, M.P, india
²Department of Botany, Govt. Geetanjali Girls College, Bhopal, M.P, India
^*Corresponding Author E-mail: mehar.sid@gmail.com
Received: 03.01.2016 | Revised: 15.01.2016 | Accepted: 19.01.2016

ABSTRACT

CMCase production by immobilized Aspergillus niger in submerged fermentation was
studied.The isolated culture of Aspergillus niger was immobilized in calcium alginate beads.
Studies were carried out on different parameters like alginate concentration, incubation
time, bead inoculum, CaCl2 concentration and curing time, which affect the productivity and
stability of the immobilized system. The maximum CMCase activity of 0.37 IU/ml was
achieved with 4% alginate concentration, 48h of incubation time, 40 beads/flask of
inoculum, 0.2M CaCl2 concentration and 60 min of curing time.
Keywords: CMCases, Calcium alginate, Aspergillus niger

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.2185

Cite this article:

Siddiqui, M., Siddiqui, N. and Varma, R., Enhanced Production of Cellulolytic Enzymes from Immobilized Cells of Aspergillus niger, Int. J. Pure App. Biosci. 4(1): 109-114 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2185

No. 772, Basant Vihar, Kota

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