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Protease from Bacillus subtilis has wide applications in industrial sectors.A total of 28 bacteria
were isolated from marine mud sample, of which 3 isolates exhibited constructive results for
protease production in skim milk, gelatin and casein agar. Among three isolates, MMK 2/8 had
superlative enzymatic activity in skim milk broth (170 U/mL) and was subjected for taxonomical
identification, which was found to be Bacillus subtilis. The finest conditions were found to be at pH
6.0, temperature 40 oC and 1% NaCl concentration and their maximal enzyme activitywas 354
U/mL, 437 U/mL and 263U/mL respectively, at 36 hrs of incubation. The molecular weight of the
protease enzyme was considered to be approximately 50 kDa by SDS-PAGE. The enzyme activity
was enriched by exposing it to ultra violet radiation [UV] for 60 secand it was observed to be 610
U/mL. Hence, we charted that the production of acidic protease treated with ultra violet radiation
was maximum when compared with the parental strain.
Keywords: Acidic protease, Bacillus subtilis, UV radiation, skim milk agar