INDIAN JOURNAL OF PURE & APPLIED BIOSCIENCES

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Archives - 2016 /Volume 4, Issue 1

Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2016 , Volume 4, Issue 1
Page No. : 172-179
Article doi: : http://dx.doi.org/10.18782/2320-7051.2193

Protease Inhibitor Associated Antimicrobial Activity of Pea Pisum sativum L. cv. Arkel

Huma Habib1*, Khalid Majid Fazili2, Mohammad Afzal Zargar3 and Bashir Ahmed Ganie4 

1Department of Biochemistry, Islamia College of Science and Commerce, Srinagar 190003, India
2Department of Biotechnology, University of Kashmir, Srinagar 190006, India
3Registrar, Central University, Srinagar, 190015, India
4Director CORD, University of Kashmir, Srinagar 190006, India
*Corresponding Author E-mail: huma99@gmail.com
Received: 17.01.2016 | Revised: 26.01.2016 | Accepted: 01.02.2016

 ABSTRACT

Aqueous and organic extracts were prepared from fresh pea seeds by standard methods. The
extracts were analyzed for protease inhibitory activity using Dot,blot assay. The aqueous as well as
the organic extracts obtained with ethanol showed significant protease inhibitory activity. The
aqueous extracts were treated with ammonium sulfate to cause differential protein precipitation.
30%, 60% and 90% ammonium sulfate saturations were used to prepare these different protein
fractions. 60% and 90% fractions were positive for protease inhibitor (PI) activity on dot blot assay,
the 30% fraction did not show any inhibitory activity. The assay of antibacterial activity by disc
diffusion method using E.coli (ATCC 25922) strain revealed that the crude aqueous extract, 90%
fraction and ethanolic extract were highly active in inhibiting the bacterial growth, the 60% fraction
showed moderate antibacterial activity and the 30% fraction was not significant.
Keywords: antibacterial activity, protease inhibitor, pisum sativum

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.2193

Cite this article:

Habib, H., Fazili, K.M., Zargar, M.A. and Ganie, B.A., Protease Inhibitor Associated Antimicrobial Activity of Pea Pisum sativum L. cv. Arkel, Int. J. Pure App. Biosci. 4(1): 172-179 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2193




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