Archives

The following study explores the antimicrobial activity, synergistic effect, potentiation effect and
phytochemical analysis of different extracts of C.buchanani leaves. C.buchanani leaves were dried
and extracted by soxhlet extraction using four different solvents viz Ethyl acetate, Methanol, Acetone
and Ethanol. The extracts of C.buchanani leaves were screened against Escherichia coli,
Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, Proteus vulgaris, Xanthomonas
citri, Citrobacter divergence, Vibrio cholerae, Candida albicans & Aspergillus niger. The
antimicrobial activity indicated that ethyl acetate extract gave maximum inhibition followed by
methanol, acetone and ethanol. Proteus vulgaris was found to be most susceptible while Aspergillus
niger was resistant to all the organic extracts for antimicrobial and synergistic activity. The
combinations of ethyl acetate extracts has shown highest synergistic effect against P.vulgaris.
Antagonistic effect was seen for fungal pathogens. Potentiation effect of Gentamicin was seen in
ethyl acetate extract against P.vulgaris. The phytochemical constituents varied significantly
depending upon the type of solvent used. Saponins and alkaloids were present in all extracts. Thus,
C.buchanani is highly rich in the diversity of phytochemical constituents.
Key words: C. buchanani, Antimicrobial activity, Synergistic effect, Potentiation effect, Phytochemicals.