INDIAN JOURNAL OF PURE & APPLIED BIOSCIENCES

ISSN (E) : 2582 – 2845

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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2016 , Volume 4, Issue 1
Page No. : 291-295
Article doi: : http://dx.doi.org/10.18782/2320-7051.2229

Microprapogation of callus from Syzygium cumini explant

S.H. Jejurker, U.R. Tanpure, S.D. Pawar, R.R. Joshi and M.S. Wadikar*

Department of Biotechnology, Vinayakrao Patil College Vaijapur, Dist. Aurangabad (M.S) 423701 India
Department of Botany, Vinayakrao Patil College Vaijapur, Dist. Aurangabad (M.S) 423701 India
*Corresponding Author E-mail: mswadikar@yahoo.com
Received: 18.02.2016 | Revised: 25.02.2016 | Accepted: 27.02.2016

 ABSTRACT

Microprapogation of the syzygium cumini explant was tried by using the different kind of MS.
medium, hormonal changes in the medium show variations in the result. At the appropriate quantity
of the hormones the explant shows a significant amount of development; the basal medium does show
the some kind of development into a bud & no effect on the leaf explant development. The auxin is
generally root initiator and cytokinin are the shoot developers. Very low level of growth regulator
concentration does not show the significant result. (0.1-mg/l -0.1 mg/l) Present work we got
development of callus by using a MS medium of basal medium + 6 BAP with additional supplement
of IBA and kinetin at concentration (0.60 mg/l – 0.60 mg/l) and at the (0.02-0.5 mg/l) of IBA &
kinetin shoot development observed. IAA only shows some growth in explant but does not develop the
tumor/ callus.
Key words: Syzygium Cumini, Explant, Basal Medium, Callus, Growth Hormones

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.2229

Cite this article:

Jejurker, S.H., Tanpure, U.R., Pawar, S.D., Joshi, R.R. and Wadikar, M.S., Micropropogation of callus from Syzygium cumini explant, Int. J. Pure App. Biosci. 4(1): 291-295 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2229




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