INDIAN JOURNAL OF PURE & APPLIED BIOSCIENCES

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Archives - 2016 /Volume 4, Issue 2

Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2016 , Volume 4, Issue 2
Page No. : 103-110
Article doi: : http://dx.doi.org/10.18782/2320-7051.2254

A Study on Inhibitory Effect of Trichoderma sp. TKD on Aspergillus flavus FNCC6109 and Its Molecular Identification

IBG Darmayasal* and I Gst Lanang Oka2

Microbiology Laboratory (FMIPA) Faculty of Mathematics and Natural Sciences, Udayana University, Indonesia
*Corresponding Author E-mail: darm_aponk@yahoo.co.id
Received: 29.03.2016 | Revised: 11.04.2016 | Accepted: 15.04.2016

 ABSTRACT

Aspergillus flavus is one type of fungus that often cause problems in the field of agriculture and
animal husbandry. Biological control is necessary in order to reduce the use of synthetic fungicides
which have good side effect on humans and the environment. Exploratory research that has been
done shows a candidate isolate potential to inhibit the growth of Aspergillus flavus. These isolates,
isolated from the rhizosphere of corn and is named Trichoderma sp. TKD. The purpose of this study
was to determine the species of Trichoderma sp. TKD and its ability in inhibiting the A. flavus
FNCC6109. Identification of Trichoderma sp. TKD was done macroscopically, microscopically and
molecularly using internal transcribed spacer region (ITS 5 F: 5 '-
¬GAAGTAAAAGTCGTAACAAGG-- 3' and ITS 4 R: 5 '- TCCTCCGCTTATTGATATGC - 3'). The
test of the inhibitory power used dual culture method. The results showed Trichoderma sp. TKD
isolated from the rhizosphere of corn was identified as Trichoderma asperellum and had the
potential to inhibit the growth of A. flavus FNCC6109 at 98.849±1.100%.
Keywords: Aspergillus flavus FNCC6109, Trichoderma sp.TKD, Aflatoxin 

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.2254

Cite this article:

Darmayasa, I.B.G. and Oka, I.G.L., A Study on Inhibitory Effect of Trichoderma sp. TKD on Aspergillus flavus FNCC6109 and Its Molecular Identification, Int. J. Pure App. Biosci. 4(2): 103-110 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2254




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