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Ayurveda research gives lot of experience to determine imbalances of three chemical substances in
our body (vatta, pitta, kapha) these three chemicals imbalance is known as tridosha , and various
types of diseases are occur due to create imbalance tridosha in our body,herbal medicine research
in ayurveda can create motive and The materia medica of the Ayurveda, composed of the five basic
elements, has been categorised according to the derivatives of these elements. They include; taste
(rasa), potency (virya), taste of the digestion product (vipaka), properties (guna) specific properties
(prabhava) and action (Karman). Finally the action (karman) of a drug on the body is expressed in
terms of the three doshas. A drug can increase or decrease the vata dosha, the pitta dosha and the
kapha dosha.as useful for determine to cure these tridosha, research chalanges to ensure quality,
safety of drug according to tridosha in our different organs of body, to discover new herbal
medicine(eg. Butea monosperma and Indigofera tinctoria) is opportunities to deal with imbalance of
tridosha and these imbalance is control as per drug decoction quantity,this shows efficacy of
drug,which is determine by cureness activity of tridosha imbalance in our body.present research
work shows standardization aspects of these two herbel medicine these are- Physicochemical tests,
Phytochemical tests,Microscopical visualisation of cells/ tissues HPTLC,Microbial culture tests.
Key words: Butea monosperma, Indigoferatinctoria, Materiamedica, HPTLC