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Gold nanoparticles biosynthesis is burgeoning day by day making an impact in all biological
applications. Green synthesis of nanoparticle has proven to be worthy due to slower kinetics, better
manipulation, and stabilization and assuring values in medicinal field due to its environmental
friendly approach and also inexpensive techniques. In the present investigation gold nanoparticles,
were synthesized using the aqueous leaf extract of Garcinia combogia as the reducing agent. The
disposition of gold nano particles were confirmed and characterized by UV–visible spectrum, X-ray
diffraction (XRD) spectroscopy, Fourier transform Infra-red spectroscopy (FTIR), Scanning electron
microscopy (SEM).Further, the antibacterial activity of the synthesized gold nano particle were
screened against Bacillus Subtilis, E. coli, L. Monocytogenes, Proteus vulgaris, Vibryo
parahaemolyticus., The gold nanoparticles also showed effective inhibitory activity against the
Bacillus licheniformis strain Daph2 with the 20 μl of concentration. Confocal laser scanning
microscopic (CLSM) images demonstrated the antibioflim potentials of AuNps concentrations
ranging from 20-40μg/ ml against B. licheniformis antibioflim activity.
Key words: Garcinia cambogia, Nanoparticles, Surface Plasmon resonance, XRD, SEM, CLSM.