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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2016 , Volume 4, Issue 3
Page No. : 21-27
Article doi: : http://dx.doi.org/10.18782/2320-7051.2296

Antioxidant, Anticancer Cell Lines and Physiochemical Evaluation of Cobra Oil

Suchitra Khunsap^1*, Taksa Vesaratchapong¹, Panithi Laongbao¹, Lawan Chanhome¹, Supranee Buranapraditkun², Narumol Pakmanee¹ and Supatsorn Boonchang¹

¹Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok 10330, Thailand
²Cellular Immunology Laboratory Allergy and Clinical Immunology Unit, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
^*Corresponding Author E-mail: sthaithumnas@yahoo.com
Received: 26.05.2016 | Revised: 12.06.2016 | Accepted: 15.06.2016

ABSTRACT

In this study, the oil was extracted from depot fat of Naja kaouthia, a cobra snake. The oil
components were detected by GC-MS which showed 26.29% of saturated fatty acid, 31.42% of
monounsaturated fatty acid and 23.74% of polyunsaturated fatty acid. The dominant components
were Palmitic acid (17.47%), Vaccenic acid (22.98%) and Linoleic acid (16.80%). Biological
functions of cobra oil were investigated for anti-oxidant and anti-cancer cells. Various
concentrations of cobra oil (20%, 10%, 5%, 2.5% and 1.25%; v/v) could inhibit free radical
scavenging by DPPH method 88.11%±1.62%, 54.84%±2.63%, 35.10%±6.41%, 14.61%±2.37% and
6.01%±3.41% respectively. In addition, KATO-III, HepG2 and SW620 cells undergo cytotoxic
activity by cobra oil for 24, 48 and 72 h detected following MTT assay. As a great IC50 of cobra oil
on KATO-III, HepG2 and SW620 were 1.63% ± 0.08% (at 72 h), 1.67% ± 0.22% (at 48 h) and 1.67%
± 0.43% (at 48 h), respectively.
Keywords: Anticancer, Antioxidant, Cobra oil, Fatty acid profile, Natural product

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.2296

Cite this article:

Khunsap, S., Vesaratchapong, T., Laongbao, P., Chanhome, L., Buranapraditkun, S., Pakmanee, N. and Boonchang, S., Antioxidant, Anticancer Cell Lines and Physiochemical Evaluation of Cobra Oil, Int. J. Pure App. Biosci. 4(3): 21-27 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2296

No. 772, Basant Vihar, Kota

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