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Antioxidants are man-made or natural substances that may prevent or delay some types of cell
damage. This study was conducted in order to determine the impact of the geographical location on
the antioxidant activity of five samples of green tea. The objective of this study was to extract the
green tea, test the antioxidant activity of green tea extracts using, to screen the phytochemical
elements using green tea extracts, as well as to study the chromatographical analysis based on the
sampling location. Five green tea from different countries was obtained from Malaysia, China,
Japan, London, and Australia. Then the green tea extracts was obtained by evaporation using rotary
evaporator. The phytochemical elements were tested for saponin, flavonoid, alkaloid, tannin and
phenol . The phytochemical screening had shown positive for all the elements tested. The antioxidant
activity was done using 1, 1 –Diphenyl-2-picrylhydrazyl (DPPH) reagent which is a stable free
radical. The antioxidant activity shown that the China green tea has the best scavenging activity of
97% while the scavenging activity of other green teas respectively are Malaysia green tea is 49% ,
Japan green tea has 77% , London green tea has 50% and Australia green tea has 70%. Finally, the
thin- layer chromatography analysis revealed the compounds of flavonoid, catechin and phenol.
This study found that there is a relationship between the geographical location and the antioxidant
activity of green tea.
Keywords: Antioxidants, cell damage, geographical location, antioxidant activity, five green tea, DPPH, scavenging activity , thin-layer chromatography , flavonoid , catechin , phenol.