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Background and objective: The current study was designed to study acute oral toxicity study of
multi-herbal formulation ECD0058 (M/S Enki Lifescience Limited, Mumbai, India) according to
OECD guidelines. For new substances it is the recommended stepwise testing approach for
developing scientifically sound data on the safety of the substance.
Conclusions: 12 female Wistar rats were selected for acute oral toxicity study of ECD0058. The
animals were fasted for minimum 16 hours prior to dosing and for 4 hours post dosing, feed was
withheld but drinking water provided ad libitum. 3 rats of the first group G1 were dosed with
starting dose of 300 mg/kg body weight and the animals did not show any mortality. So another 3
animals of the same group G1 were dosed with 300 mg/kg body weight and no mortality was
observed. Next, 3 animals of group G2 were dosed with 2000 mg/kg body weight and no mortality
was observed. So, another 3 animals of the same group G2 were dosed with 2000 mg/kg body weight
and no mortality was observed. Hence, further dosing was stopped.
At 300 and 2000 mg/kg body weight, all the animals were observed normal throughout the
experimental period.
Keywords: Enki Lifescience Limited, Toxicity, Rat