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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2016 , Volume 4, Issue 3
Page No. : 97-103
Article doi: : http://dx.doi.org/10.18782/2320-7051.2303
In Vitro and In Vivo Induction, and Characterization of Toxins Isolated from Beauveria bassiana
A.G. Vikhe*, N.S.Dale, R.B. Umbarkar, G.B. Labade, A.R.Savant, and A.A. Walunj
1Research Scholar, 2Associate Professor of Entomology, 3Associate Professor of Agronomy, 4Assistant Professor of Botany, 5Assistant Professor of Plant Biotechnology,
6Assistant Professor of Plant Pathology and Microbiology Department of Agril. Entomology, College of Agriculture and Agril. Biotechnology, LONI-413736 Tal- Rahata, Dist-Ahmednagar, Maharashtra India
*Corresponding Author E-mail: akshay.vikhe99@gmail.com
Received: 5.06.2016 | Revised: 19.06.2016 | Accepted: 23.06.2016
ABSTRACT
Entomopathogenic fungi produce secondary metabolites which may bio actively help fungus in its
virulence toward insect hosts. Beauveria bassiana produces several toxic low molecular compounds
in vitro as well as in vivo, the most important of them is Beauvericin. The Beauveria bassiana
fungus isolated was selected for Toxin assay. Various Toxins was obtained from surface and
submerged cultures of the fungus in PDYB and PDA, culture filtrates, and in vivo conidia harvested
from insect cadavers. Results indicated that in vivo fungal conidia contained the most Beauvericin,
causing a higher mortality compared with in vitro fungal products in their different concentrations.
Beauvericin chromatogram revealed that Beauvericin was in its greatest quantity in comparison
with the other secondary metabolites of Beauveria bassiana isolate. The impact of Beauvericin on
mean and on paralysis time was in agreement with bioassay data in treated with metabolites of the
insect-derived conidia.
Keywords: Beauveria bassiana , HPLC, MALDI-TOF, Toxin Identification.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.2303
Cite this article:
Vikhe, A.G., Dale, N.S., Umbarkar, R.B., Labade, G.B., Savant, A.R. and Walunj, A.A., In Vitro and In Vivo Induction, and Characterization of Toxins Isolated from Beauveria bassiana , Int. J. Pure App. Biosci. 4(3): 97-103 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2303
