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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2016 , Volume 4, Issue 3
Page No. : 130-135
Article doi: : http://dx.doi.org/10.18782/2320-7051.2304
Isolation and Purification of Cuticle Degrading Extra Cellular Proteases from Entomopathogenic Fungal Species of Beauveria bassiana
A.G. Vikhe1*, N.S. Dale2, R.B. Umbarkar3, G.B. Labade4, A.R. Savant5, and A.A. Walunj6
1Research Scholar, 2Associate Professor of Entomology, 3Associate Professor of Agronomy, 4Assistant Professor of Botany, 5Assistant Professor of Plant Biotechnology, 6Assistant Professor of Plant Pathology and Microbiology Department of Agril. Entomology, College of Agriculture and Agril. Biotechnology, LONI-413736 Tal- Rahata, Dist-Ahmednagar, Maharashtra India
*Corresponding Author E-mail: akshay.vikhe99@gmail.com
Received: 5.06.2016 | Revised: 16.06.2016 | Accepted: 18.06.2016
ABSTRACT
The entomotoxic protein of Beauveria bassiana (Bals.) (Ascomycota:Hypocreales) was precipitated
with 90% saturated (NH4)2SO4 and the precipitate was eluted in Sephadex G-25 column. The
protein content was higher (0.026 μg/ml) supported by UV spectroscopy absorbance. The vibration
frequency in FT-IR appeared between the ranges of 1650– 1580cm-1 due to N-H bond formation for
amines. Bioassays of Beauveria bassiana reveal that caused significantly higher insecticidal
activity.Amylase, Protease and Lipase (digestive enzymes), Esterase, Glutathione S-Tranferase and
Lactate dehydrogenase level/activity in insects. Hence, the Beauveria bassiana was subjected to
sodium dedosyl sulphate–poly-acrylamide gel electrophoresis (SDS-PAGE)
Keyword: Beauveria bassiana, Protein Extraction, FT-IR, SDS-PAGE
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.2304
Cite this article:
Vikhe, A.G., Dale, N.S., Umbarkar, R.B., Labade, G.B., Savant, A.R. and Walunj, A.A., Isolation and Purification of Cuticle Degrading Extra Cellular Proteases from Entomopathogenic Fungal Species of Beauveria bassiana, Int. J. Pure App. Biosci. 4(3): 130-135 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2304
