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Background:
Pseudomonas aeruginosa is a nosocomial pathogen distributed worldwide.This study examined the
antimicrobial resistance patterns of P. aeruginosa clinical isolates procured from hospitalized
patients.
Aims and objectives:
To study the Resistance pattern of Pseudomonas aeruginosa isolates from miscellaneous samples.
Materials and methods:
A total number of 125 Pseudomonas strains were isolated from 904 miscellaneous samples (Sputum,
HVS, Pus, Ear swab) between January 2016 to March 2016. Samples were obtained from the
hospitals and processed in our central Microbiology lab. Antimicrobial susceptibility test were
performed by Kirby Bauer disc diffusion method as per the recommendations of NCCLS guidelines.
Result:
P. aeruginosa were isolated from125 miscellaneous samples such as sputum (49%) followed by HVS
(27%), pus and wound swab (22%) & ear swab(2%). Most effective antibiotic was the
Aminoglycosides drugs– Amikacin (93.6%), Gentamicin(76%), followed by Carbapenem drug-
Imipenem (80%) and Fluoroquinolones drug-Ciprofloxacin (72.8%).Our results reveal high
resistance to Penicillin drug- Amoxicillin (95.2%) and 1st, 3rd generation Cephalosporins
(Cefazoline (92.8%), Cefotaxime (88.8%)).
Conclusion:
This study implicates that, the organism isolated from various clinical specimens were highly
sensitive to Amikacin, which is followed by Imipenem, Gentamicin, Ciprofloxacin. In hospitalized
patients, the emergence and spread of the resistance can be reduced by regular monitoring of drug
resistance and strict infection control measures should be ensured.
Key words: Antimicrobial resistance, Clinical isolates, Pseudomonas aeruginosa