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Two coastal areas marine water and soil samples were collected from Vedharanyam and Muthupet of Thiruvarur and Nagapattinam District respectively. Marine Bacillus were isolated and identified according to morphology, biochemical test and colony characters on selective Marine agar medium. Marine Bacillus isolates were Bacillus licheniformis, Bacillus pumilus, Bacillus subtilis and Bacillus cereus. The TLC autobiographic overlay assay implied that the antibacterial activities produced by four strains with wide antibacterial spectrum. The isolates were tested for antibacterial activity against the terrestrial pathogens namely Salmonella typhi, Staphylococcus aureus, Escherichia coli and Enterobacter aerogenes. Bacillus licheniformis, Bacillus pumilus, Bacillus subtilis and Bacillus cereus were showed clear zone of inhibition against test organisms. The maximum antibacterial activity was noted in Bacillus subtilis (21.5mm) against Escherichia coli. The minimum antibacterial activity was recorded in Bacillus pumilus (13mm) against Escherichia coli.
Keywords: Marine Bacillus, Antibiotic, Antibacterial activities , Terrestrial Pathogens, Thin layer chromatography.