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Amylases are amongst most widely used enzymes in industries such as food, fermentation, starch
processing, textile and paper. In the present investigation, bacteria were isolated from rhizosphere of Rice Agricultural Experimental Farm, Giridh, Jharkhand, screened for the production of amylase and their optimum growth conditions were determined. A total of 36 bacterial colonies were isolated from collected soil samples. Thirty Four isolates are from geneus Bacillus and two are paenibacillus, out of these five bacterial isolates, displayed zones of clearance in starch hydrolysis test. The isolate displaying maximum amylase activity on quantization was selected. Characteristic feature of the strain indicates that it belongs to the genus Bacillus and will be later used for further characterization. Maximum yield of amylase was obtained after 48hrs of incubation. The optimum pH for enzyme activity was found to be at pH 7.0 and the optimum temperature for the activity was found to be at 40oC.
Key words: Amylase, Starch Hydrolysis, characterization, amylase activity