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Background and Aim: In-vitro effrct of various growth regulators on propagation of Plumbago Zeylanica L., a medicinally herbs of India was observed in this study. C. Paniculatus has been used in the various ancient traditional system.
Materials and Method: Healthy nodal segments of the herb was used as explants with basic MS medium for shoot initiation and multiplication containing various combinations of different growth regulators. MS full and 1/2MS were used for rooting of plantlets with 25 to 200 mg/l Activated Charcoal (AC).
Result: Maximum mean number of initiated plantlets 1.7 ± 0.48 with mean length 2.7 ± 0.82 were found in MS medium treated with lowest concentration of BA (0.44 μM), while maximum mean number of multiplied plantlets 19.4 ± 0.96 with mean length 6 ± 0.94 were found in MS medium treated with 4.44 μM. Maximum mean number of roods 12 ± 1.56 with Mean length 8.92 ± 0.75 was observed on 1/2MS medium with 125 mg/l activated charcoal. The rooted plantlets were successfully hardened in 1:1:1 ratio of sand: soil: vermicompost and successfully established in soil.
Keywords: Plumbago Zeylanica L., Micropropagation, Medicinal Plants, Plant Tissue Culture.