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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2017 , Volume 5, Issue 1
Page No. : 844-848
Article doi: : http://dx.doi.org/10.18782/2320-7051.2476

Detection of GFP Expression and Colonization of Wheat by two Endophytic Bacteria tagged with GFP Gene

Disheeta L. Akbari^1* and L. F. Akbari²

¹Department of Biotechnology and 2Department of Plant Pathology, Junagadh Agricultural University, Junagadh-362001, Gujarat, India
*Corresponding Author E-mail: disheetaakbari@gmail.com
Received: 19.01.2017 | Revised: 30.01.2017 | Accepted: 1.02.2017

ABSTRACT

The two endophytic bacteria had 92% similarity with Pseudomonas synxantha strain NBRC103159 and 96% with Pseudomonas cedrina strain CFML96-198 were identified using 16S ribosomal DNA sequence and isolated from stems of Eleusine indica. Its endophytic colonization were investigated microscopically using green fluorescent protein introduced by vector pGLO containing ampicillin resistance gene and arabinose sugar. The strains were able to colonize wheat tissues and localized in the roots, stems and leaves.
Keywords: Pseudomonas spp., pGLO vector, GFP, Fluorescent microscopy, Wheat

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.2476

Cite this article:

Akbari, D.L. and Akbari, L.F., Detection of GFP Expression and Colonization of Wheat by two Endophytic Bacteria tagged with GFP Gene, Int. J. Pure App. Biosci. 5(1): 844-848 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2476

No. 772, Basant Vihar, Kota

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